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Sample Requirements
Please take some time to review our sample requirements. By following these guidelines you increase the quality of your results and you give us the possibility to help you further.
Concentration
| Template | Concentration |
| Plasmid | 40-100 ng/µl |
|
PCR (purified and unpurified products)*
PCR 1000 - 2000 bp
PCR 500 - 1000 bp
PCR 250 - 500 bp
PCR 100 - 250 bp
|
General Rule: 1.5 ng/µl per 100 bp
30 ng/µl
15 ng/µl
7.5 ng/µl
4 ng/µl
|
|
E.coli
|
150 µl LB Medium OR
visible colony on agar plate
|
| E.coli (Ecoli NightSeq®) | add 1 colony (diameter 1 mm) OR add 5 ul turbid liquid culture |
*Upfront PCR purification is highly recommended but can easily be outsourced to Microsynth.
|
Primer
|
Concentration
|
| Premixed | 4 pmol/µl = 4 µM |
|
As separate tube (enclosed)
|
10 pmol/µl = 10 µM
|
Volumes
| Service | Volume | Additional Information |
|
Economy Run (Tube)
|
12 µl
|
Premixed option: 12 µl DNA template solution + 3 µl 20µM sequencing primer solution
|
|
Economy Run (Plate)
|
10 µl/well in 96-well plate
|
Increase the volume by 5 µl /well for each additional reaction
|
|
Premium Run
|
At least 20 µl
|
Increase the volume by 5 µl for each additional reaction
|
|
Primer Walking Service
|
At least 20 µl
|
Single Stranded 0.5 µg of plasmid-DNA for every kb of your construct Double stranded 1 µg of plasmid-DNA for every kb of your construct |
| Primer | Volume |
| As separate tube (enclosed) | 20 µl + 3 µl per additional reaction |
Storage
|
Service
|
Storage at Microsynth |
|
Economy Run (Tube)
|
4 days
|
|
Economy Run (Plate)
|
1 month
|
|
Ecoli NightSeq® (Tube or Plate)
|
1 month
|
|
Premium Run
|
3 months
|
|
Primer Walking Service
|
3 months
|
|
Primer (stored)
|
6 months
|
|
Primer (custom primer list)
|
12 months
|