Back to top
Sample Requirements
Please take some time to review our sample requirements. By following these guidelines you increase the quality of your results and you give us the possibility to help you further.
Concentration
| Template | Concentration |
| Plasmid | 40-100 ng/µl |
|
PCR (purified and unpurified products)
PCR 1000 - 2000 bp
PCR 500 - 1000 bp
PCR 250 - 500 bp
PCR 100 - 250 bp
|
General Rule: 1.5 ng/µl per 100 bp
30 ng/µl
15 ng/µl
7.5 ng/µl
4 ng/µl
|
| E.coli |
150 µl LB Medium OR
visible colony on agar plate
|
|
Primer
|
Concentration
|
|
As separate tube (enclosed)
|
10 µM
|
|
Premixed
|
2 µM
|
Volumes
| Service | Volume | Additional Information |
|
Economy Run and Barcode Economy Run
|
12 µl
|
Premixed option: 12 µl DNA template solution + 3 µl 10µM sequencing primer solution
|
|
High-Throughput and Barcode High-Throughput Run
|
10 µl/well in 96-well plate
|
Increase the volume by 5 µl /well for each additional reaction
|
|
Premium Run
|
At least 20 µl
|
Increase the volume by 5 µl for each additional reaction
|
|
Primer Walking Service
|
At least 20 µl
|
Single Stranded 0.5 µg of plasmid-DNA for every kb of your construct Double stranded 1 µg of plasmid-DNA for every kb of your construct |
| Primer | Volume |
| As separate tube (enclosed) | 20 µl + 3 µl per additional reaction |
Storage
|
Service
|
Storage at Microsynth |
|
Economy Run and Barcode Economy Run
|
4 days
|
|
High-Throughput and Barcode High-Throughput Run
|
1 month
|
|
Premium Run
|
3 months
|
|
Primer Walking Service
|
3 months
|
|
Primer (stored)
|
6 months
|
|
Primer (custom primer list)
|
12 months
|