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Sample Requirements

 
 
Please take some time to review our sample requirements. By following these guidelines you increase the quality of your results and you give us the possibility to help you further.
 
 

Concentration

 
Template Concentration
Plasmid 40-100 ng/µl
PCR (purified and unpurified products)
 
PCR 1000 - 2000 bp
PCR  500 - 1000 bp
PCR   250 - 500 bp
PCR   100 - 250 bp
General Rule: 1.5 ng/µl per 100 bp
 
30 ng/µl
15 ng/µl
7.5 ng/µl
4 ng/µl
E.coli
150 µl LB Medium OR
visible colony on agar plate
E.coli (Ecoli NightSeq®) add 1 colony (diameter 1 mm) OR
add 5 ul turbid liquid culture
 
Primer
Concentration
Premixed 4 pmol/µl = 4 µM
As separate tube (enclosed)
10 pmol/µl = 10 µM

Volumes

 

Service Volume Additional Information
Economy Run (Tube)
12 µl
Premixed option: 12 µl DNA template solution + 3 µl 20µM sequencing primer solution
Economy Run (Plate)
10 µl/well in 96-well plate
Increase the volume by 5 µl /well for each additional reaction
Premium Run
At least 20 µl
Increase the volume by 5 µl for each additional reaction
Primer Walking Service
At least 20 µl

Single Stranded

0.5 µg of plasmid-DNA for every kb of your construct

 

Double stranded

1 µg of plasmid-DNA for every kb of your construct

Primer Volume
As separate tube (enclosed) 20 µl + 3 µl per additional reaction

 

Storage

 

Service
Storage at Microsynth
Economy Run (Tube)
4 days
Economy Run (Plate)
1 month
Ecoli NightSeq® (Tube or Plate)
1 month
Premium Run
3 months
Primer Walking Service
3 months
Primer (stored)
6 months
Primer (custom primer list)
12 months