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Microbial Resequencing and Profiling
- Check or validate mutations on the genome level
- Validate your bacterial production stem
- Characterize microbial isolates by MLST
Considerations before starting a microbial resequencing project:
- Is there a reference genome?
- Is there a MLST profile?
- Are larger modifications expected?
- May a substantial DNA contamination occur?
Let us guide you – from design to analysis
Example projects using microbial resequencing:
- Detect the pathogenicity of bacterial DNA isolates by MLST
- Verify/detect SNPS and InDels
- Check for the specificity of an introduced mutation
Applications related to microbial resequencing:
- Amplicon metagenomics
- De novo sequencing
- Plasmid sequencing
In bacterial resequencing studies a bacterial strain is sequenced and compared against the sequence of a reference strain. Our bacterial resequencing analysis module helps you to answer the following questions:
- How high is the sequencing coverage for the resequenced genome? (see Figure 1)
- What differences in the genomic structure such as single nucleotide variations (SNVs) or small insertions and deletions (InDels) are present and what is their position within the genome? (see Table 1)
- How do the observed mutations affect annotated genes and the corresponding protein sequence? (see Table 2)
Table 2: Table displaying detailed information on each observed variation like variant type and position, affected protein including reference and altered sequence.