Innovative new Sanger sequencing service in 1.5 ml tubes for plasmids. Drop your E. coli colonies into a Microsynth drop box, receive your result the next day before 2 pm and accelerate your research by one day.
Features and Benefits
Additionally to the features and benefits you know from the Barcode Economy Run, the Ecoli NightSeq offers following benefits:
Overnight service, delivery of results before 2 pm the next day
Results are delivered Monday to Friday
Use our drop box in your vicinity for free shipping of samples
You can choose between prepaid and non-prepaid service
Accelerate your research by one working day
Save handling time in the lab
Significantly reduce your expenditures on plasmid DNA purification kits by only isolating plasmids from clones showing the desired sequence
The Ecoli NightSeq service is also available in the plate format.
Click on Fill Order Form under Ecoli NightSeq (prepaid or non-prepaid) and follow the further instructions
See also Microsynth's user guide on the right side for more detailed information.
Do I have to add antibiotics to the liquid?
No, an antibiotic is not necessary.
Do I really have to pick from a colony that is at least 1 mm in diameter?
The colony may be a bit smaller, but it must be clearly visible.
I want to inoculate the Ecoli NightSeqTM tubes and streak out on an agar plate with the same toothpick. What should I do first: inoculate the ENS tube or streak out my agar plate?
First the ENS tube.
Does ENS work for low copy plasmids as well?
I do not work with agar plates, but with liquid culture. Can I also inoculate the ENS Tubes with liquid culture?
Yes, please add 5 µl.
For how long does Microsynth store my ENS samples?
How long can I store the unused tubes with liquid? Under what conditions do I have to store them?
On the plastic bag there is a sticker with information about the expiry date (1 year) and the storage temperature (4° C for single tubes, -20 ° C for plates).
I would like to pick a clone and do a forward and a reverse reaction on it. Is that possible?
Yes, but you must use 2 barcoded tubes. The procedure is as follows: Inoculate one tube and mix and divide the fluids of both tubes.
What you should not do: Inoculate one tube and then inoculate the other tube with the same toothpick, because then you have an unequal cell density.
I do not have a Microsynth drop box in my vicinity, may I also mail it, even if it stays over the weekend?
Yes. We have tested a correspondingly long storage at room temperature and it doesn’t harm the sample.
Do I receive the results on Saturday, when I drop the samples on Friday into the drop box?
No, only on Monday before 2 pm.
How should I send / label the sequencing primer?
The same way as with our other types of services.
Concentration 10 µM, at least 20 µl volume and write the name of the primer on the tube.
What do I need to consider when shipping living GMOs to Microsynth Seqlab?
Genetically modified microorganisms (GMOs) that do not meet the definition of toxic or infectious substances, but are able to modify animals, plants or microbial substances in a way that does not normally result from natural reproduction, do belong to Class 9 and shall be assigned to UN3245 and shipped in accordance with Packing Instruction P904. In short, this means the samples must be packed in an inner, a secondary and an outer packaging. The inner packaging comprises of a leakproof primary receptacle and an absorbent material placed between the primary receptacle and the secondary packaging (e.g. plastic bag). The outer packaging shall be strong enough for its capacity, mass and intended use and shall have a minimum outer dimension of at least 100 mm. Further information can be found at https://adrbook.com/en/2017/Sutun/8/P904.
If GMOs cannot be assigned to UN3245/class 9 (which is true for most E. coli laboratory strains and vectors), they are not subject to dangerous goods regulations.
GMOs are not subject to the dangerous goods regulations if they are approved for use by the competent authorities of the countries of origin, transit and destination.
“The main benefits of Ecoli NightSeq are saving one day as well as saving time and consumable costs for the mini-prep of 3-5 picked colonies from each clone. Another benefit is that Microsynth Seqlab provides the option for standard primers, which makes life easier for many constructs. In terms of quality, whenever poor sequence reads were found, Microsynth Seqlab has repeated the sequencing with no extra cost. Taken together, I’m extremely satisfied with the new Ecoli NightSeq service, an additional high standard sequencing service from Microsynth Seqlab.”
Dr. Shubhendu Palei, TU Dortmund
“Thanks to the new Ecoli NightSeq service from Microsynth, we are now able to sequence in high-throughput format and save up to two days compared to the previous service. Time really is money for us since we can significantly shorten critical project timelines. The easy sample preparation, the high-quality sequences, the long read-lengths as well as the competent, fast and uncomplicated customer support were convincing us, too.”
Andreas Lehmann, Molecular Partners AG
“We’ve been using the Ecoli NightSeq service proposed by Microsynth for 6 months now and have only seen advantages to the system. It allows you to save one day in your cloning process by sending directly your colonies for sequencing. The most important aspect is that you do not need to extract the DNA saving bench time and money. This reduces in effect the general price of the read. We’ve been surprised by the quality of the reads which you cannot distinguish from extracted DNA reads. Overall, it’s a plus!”
Thomas Lunardi, EPFL SV ISREC UPLIN
“I have used the Ecoli NightSeq service primarily to test whether a site directed mutagenesis has worked, as well as to check cloned CrisprCas9 plasmids. For such applications this service is very convenient, as they need to be confirmed with sequencing anyways and hence Ecoli NightSeq allowed me to save unnecessary minipreps.”
Barbara Hauert, University of Bern