Innovative new Sanger sequencing service in 1.5 ml tubes for plasmids. Drop your E. coli colonies into a Microsynth drop box, receive your result the next day before 2 pm and accelerate your research by one day.
Features and Benefits
Additionally to the features and benefits you know from the Barcode Economy Run, the Ecoli NightSeq offers following benefits:
Overnight service, delivery of results before 2 pm the next day
Results are delivered Monday to Friday
Use our drop box in your vicinity for free shipping of samples
You can choose between prepaid and non-prepaid service
Accelerate your research by one working day
Safe handling time in the lab
Significantly reduce your expenditures on plasmid DNA purification kits by only isolating plasmids from clones showing the desired sequence
The Ecoli NightSeq service is also available in the plate format.
Click on Fill Order Form under Ecoli NightSeq (prepaid or non-prepaid) and follow the further instructions
See also Microsynth's user guide on the right side for more detailed information.
Do I have to add antibiotics to the liquid?
No, an antibiotic is not necessary.
Do I really have to pick from a colony that is at least 1 mm in diameter?
The colony may be a bit smaller, but it must be clearly visible.
I want to inoculate the Ecoli NightSeqTM tubes and streak out on an agar plate with the same toothpick. What should I do first: inoculate the ENS tube or streak out my agar plate?
First the ENS tube.
Does ENS work for low copy plasmids as well?
I do not work with agar plates, but with liquid culture. Can I also inoculate the ENS Tubes with liquid culture?
Yes, please add 5 µl.
For how long does Microsynth store my ENS samples?
How long can I store the unused tubes with liquid? Under what conditions do I have to store them?
On the plastic bag there is a sticker with information about the expiry date (1 year) and the storage temperature (4° C for single tubes, -20 ° C for plates).
I would like to pick a clone and do a forward and a reverse reaction on it. Is that possible?
Yes, but you must use 2 barcoded tubes. The procedure is as follows: Inoculate one tube and mix and divide the fluids of both tubes.
What you should not do: Inoculate one tube and then inoculate the other tube with the same toothpick, because then you have an unequal cell density.
I do not have a Microsynth drop box in my vicinity, may I also mail it, even if it stays over the weekend?
Yes. We have tested a correspondingly long storage at room temperature and it doesn’t harm the sample.
Do I receive the results on Saturday, when I drop the samples on Friday into the drop box?
No, only on Monday before 2 pm.
How should I send / label the sequencing primer?
The same way as with our other types of services.
Concentration 10 µM, at least 20 µl volume and write the name of the primer on the tube.